The Correlation of Single Nucleotide Polymorphism of the ACE2 rs4646116 Gene with The Severity of COVID-19

SUMMARY. Different genetic and immunological factors can affect the severity of Coronavirus disease 19. Angiotensin-converting enzyme 2 is a human receptor for Severe Acute Respiratory Syndrome Coro-navirus-2, and the successful interaction between the spike protein of the novel virus and Angiotensin-converting enzyme 2 is responsible for the initial and complete infection. The study aimed to evaluate the correlation between Single Nucleotide Polymorphisms of Angiotensin converting-enzyme 2, with disease severity of Coronavirus disease 19 in AL-Najaf province. The allele Specific-polymerase Chain reaction method was used for investigating Single Nucleotide polymorphisms of Angiotensin converting-enzyme 2 rs4646116 A/G in different states of Coronavirus disease 19 (COVID-19). The wild genotypes (GG) for ACE2 rs4646116 gene recorded a highly significant association p = 0.0009, and a high ratio in the control group (90%) in comparison with moderate cases of COVID-19 (60%). While the heterozygote genotype (GA) of the same gene showed a significant (p-value = 0.0144) and high ratio in moderate cases (30%) in comparison with the control group (10%). Conclusion(s): the wild genotype (GG) for Angiotensin convert-ing-enzyme 2 rs4646116 gene may be associated with more protection from infection with COVID-19. While the polymorphism heterozygote genotype (GA) for the same gene may be associated with more susceptibility to infection with COVID-19.Copyright © 2023, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.

The Role of ACE2 rs41303171 Gene Polymorphism in Susceptibility for Infection with SARS-CoV-2

Background: Angiotensin-converting enzyme 2 (ACE2) is a human receptor for Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2), and successful interactions between the spik protein of SARS- CoV-2 and ACE2 are responsible for the initial and complete infection. The aim of the current study included: The determination of Single Nucleotide Polymorphisms A/G of the ACE2 rs41303171 gene and their association with the susceptibility to infection with SARS-CoV-2. Material and Methods: Allele Specific-polymerase Chain reaction (AS-PCR) method was used for investigating Single Nucleotide polymorphisms A/G of ACE2 rs41303171 in different states of patients with COVID-19, Results: The wild genotype of (GG) for the ACE2 rs41303171 gene may be more susceptible to the infection with SARS-CoV-2. In contrast, the heterozygote genotype (AG) and mutated genotype (AA) for ACE2 rs41303171gene may be associated with more protection from the infection with SARS-CoV-2. Conclusion: The main findings of our study show an apparent positive correlation between susceptibility to infection and severity of COVID-19 with the qualitative expression of the ACE2 gene in Iraqi people. © 2022, ResearchTrentz Academy Publishing Education Services. All rights reserved.